Prenatal Sample Requirements

Baylor Genetics accepts the following sample types for prenatal tests. Submitted sample types must meet the sample requirements for all ordered prenatal testing. For assistance with billing questions, kit requests, and/or shipping, please call 1-800-411-4363. Genetic counselors may be reached via email at gc@baylorgenetics.com for test inquiries. Shipping and labeling instructions can be found at the bottom of the page.

Prenatal Cytogenetic Testing

Prenatal Tests	Specimen Type	Quanity Required			Additional Required Samples
		Direct	Cultured	Extracted DNA
Prenatal  Chromosome Analysis, Array Analysis, or Array + Limited Chromosome Analysis	CVS Test Codes: 8700, 8657, 8671, 8672, 8676	15+ mg CVS in sterile tissue culture media	1 T-25 flask at about 80% confluency	For Array Analysis Only: 20ug of purified DNA (minimal concentration of 50ng/uL; A260/A280 of ~1.7)	For Array Analysis Only: Maternal required and paternal preferred for array analysis
	Amniotic Fluid Test Codes: 8530, 8656, 8670, 8673, 8675	20-30 cc of fluid in two sterile 15 mL conical centrifuge tubes. Discard the first 2 cc of fluid collected.	1 T-25 flask at about 80% confluency
FISH Analysis – Prenatal Aneuploidy Test Code: 8410	CVS	15+ mg CVS in sterile tissue culture media	N/A	N/A	N/A
	Amniotic Fluid	20-30 cc of fluid in two sterile 15 mL conical centrifuge tubes. Discard the first 2 cc of fluid collected.	N/A	N/A

Prenatal Whole Exome Sequencing

Prenatal Tests	Specimen Type	Quanity Required			Additional Required Samples
		Direct	Cultured	Extracted DNA
Prenatal Trio Whole Exome Sequencing Test Code: 1622	CVS	15+ mg CVS in sterile tissue culture media	2 T-25 flask at about 80% confluency from separate primaries	20ug of purified DNA (minimal concentration of 50ng/uL; A260/A280 of ~1.7)	Maternal and paternal required
	Amniotic Fluid	20-30 cc of fluid in two sterile 15 mL conical centrifuge tubes. Discard the first 2 cc of fluid collected.	2 T-25 flask at about 80% confluency from separate primaries

Prenatal Molecular

Prenatal Tests	Specimen Type	Quanity Required			Additional Required Samples
		Direct	Cultured	Extracted DNA
Custom Sequence Analysis Test Code: 1522	CVS	15+ mg CVS in sterile tissue culture media	2 T-25 flask at about 80% confluency from separate primaries	20ug of purified DNA (minimal concentration of 50ng/uL; A260/A280 of ~1.7)	Maternal and positive control required
	Amniotic Fluid	20-30 cc of fluid in two sterile 15 mL conical centrifuge tubes. Discard the first 2 cc of fluid collected.	2 T-25 flask at about 80% confluency from separate primaries
DMD Deletion/Duplication Test Code: 6351	CVS	15+ mg CVS in sterile tissue culture media	2 T-25 flask at about 80% confluency from separate primaries		Maternal and positive control required
	Amniotic Fluid	20-30 cc of fluid in two sterile 15 mL conical centrifuge tubes. Discard the first 2 cc of fluid collected.	2 T-25 flask at about 80% confluency from separate primaries
Myotonic Dystrophy Type 1 Repeat Expansion Analysis Test Code: 6105	CVS	15+ mg CVS in sterile tissue culture media	2 T-25 flask at about 80% confluency from separate primaries		Maternal and positive control required
	Amniotic Fluid	20-30 cc of fluid in two sterile 15 mL conical centrifuge tubes. Discard the first 2 cc of fluid collected.	2 T-25 flask at about 80% confluency from separate primaries
Huntington Disease Repeat Expansion Analysis Test Code: 6099	CVS	15+ mg CVS in sterile tissue culture media	2 T-25 flask at about 80% confluency from separate primaries		Maternal and positive control required
	Amniotic Fluid	20-30 cc of fluid in two sterile 15 mL conical centrifuge tubes. Discard the first 2 cc of fluid collected.	2 T-25 flask at about 80% confluency from separate primaries
FMR1 (Fragile X) CGG Repeat Expansion Analysis Test Code: 6574	Amniotic Fluid
		20-30 cc of fluid in two sterile 15 mL conical centrifuge tubes. Discard the first 2 cc of fluid collected.	2 T-25 flask at about 80% confluency from separate primaries		Maternal required and paternal preferred
MECP2 (Rett Syndrome) Deletion/Duplication Test Code: 6109	CVS	15+ mg CVS in sterile tissue culture media	2 T-25 flask at about 80% confluency from separate primaries		Maternal and positive control required
	Amniotic Fluid	20-30 cc of fluid in two sterile 15 mL conical centrifuge tubes. Discard the first 2 cc of fluid collected.	2 T-25 flask at about 80% confluency from separate primaries
IKBKG (Incontinentia Pigmenti) Common Deletion Analysis Test Code: 6100	CVS	15+ mg CVS in sterile tissue culture media	2 T-25 flask at about 80% confluency from separate primaries		Maternal and positive control required
	Amniotic Fluid	20-30 cc of fluid in two sterile 15 mL conical centrifuge tubes. Discard the first 2 cc of fluid collected.	2 T-25 flask at about 80% confluency from separate primaries
Prenatal Noonan Spectrum Disorders/RASopathy Panel Test Code: 24001-P12-05	CVS	15+ mg CVS in sterile tissue culture media	2 T-25 flask at about 80% confluency from separate primaries		Maternal required
	Amniotic Fluid	20-30 cc of fluid in two sterile 15 mL conical centrifuge tubes. Discard the first 2 cc of fluid collected.	2 T-25 flask at about 80% confluency from separate primaries

Other Prenatal

Prenatal Tests	Specimen Type	Quanity Required			Additional Required Samples
		Direct	Cultured	Extracted DNA
Alpha-fetoprotein and Acetylcholinesterase Analysis Test Code: 8550	Amniotic Fluid	Collect at least 3 mL amniotic fluid in a sterile leak-proof container.
			N/A	N/A	N/A
Alphafetoprotein Analysis Test Code: 8501	Amniotic Fluid	Collect at least 3 mL amniotic fluid in a sterile leak-proof container.
			N/A
Herpes Simplex Virus 1 & 2 Analysis Test Code: 8940	Amniotic Fluid	Collect 1 mL amniotic fluid in a sterile leak-proof container.
			N/A
Cytomegalovirus (CMV) Analysis Test Code: 8945	Amniotic Fluid	Collect 1 mL amniotic fluid in a sterile leak-proof container.
			N/A
Toxoplasma gondii (TOXO) Analysis Test Code: 8950	Amniotic Fluid	Collect 1 mL amniotic fluid in a sterile leak-proof container.
			N/A
Parvovirus B19 Analysis Test Code: 8955	Amniotic Fluid	Collect 1 mL amniotic fluid in a sterile leak-proof container.
			N/A

Products of Conception (POC)

Tests	Specimen Type	Quanity Required			Additional Required Samples
		Direct	Cultured	Extracted DNA
Chromosome Analysis Test Code: 8800   and   Chromosomal Microarray Analysis Test Code: 8639
	Unclotted Cord or Cardiac Blood	For Chromosome Analysis: Draw 3-5 cc blood in Sodium Heparin (green-top) tube(s).For Array Analysis: Draw 3-5 cc blood in an EDTA (purple-top) tube(s).
			N/A	For Array Analysis Only: 20ug of purified DNA (minimal concentration of 50ng/uL; A260/A280 of ~1.7)	For Array Analysis Only: Maternal and paternal preferred
	Placenta	Collect 10 cubic millimeters placenta from fetal side near the site of cord insertion. Place sample in a separate sterile container with RPMI media. In the absence of RPMI media, place sample in a sterile container with a small amount of sterile saline. Never place samples in formalin or other fixative.
			N/A
	Identifiable Fetal Parts	Collect 5 cubic millimeters of sample in a separate sterile container with RPMI media. In the absence of RPMI media, place sample in a sterile container with a small amount of sterile saline. Never place samples in formalin or other fixative.
			N/A
	Chorionic Villi or Non-Fixed Tissue	Please send at least 50-100 mg villi or 0.5 – 1 cm3 tissue(s) in a sterile container with either sterile: 1. Transport media provided by our laboratory, 2. Ringer’s lactate, or 3. Hanks’ balanced salt solution.
			N/A
	Cultured Skin Fibroblast	N/A
			1-2 T25 flasks at 80-100% confluence.
	Cultured Tissue	N/A
			1-2 T25 flasks at 80-100% confluence.
Other
	Other	Please contact the laboratory to discuss other test codes and specimen types that may be acceptable for products of conception.

Shipping Instructions:
Ship the sample at room temperature to the laboratory by overnight express. Specimen should arrive in the laboratory within 48 hrs of sample date. Do not heat or freeze.

Labeling:
For prenatal testing, please make sure that the specimen containers have two identifiers that match the prenatal test requisition. Identifiers include, but are not limited to, the following:

Patient’s First and Last Name
Date of Birth
Accession #
Hospital / Medical Record #